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PMPCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89126

Novus Biologicals, part of Bio-Techne
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NBP1-89126-25ul
NBP1-89126

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNVSLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PMPCA Antibody

Immunohistochemistry-Paraffin: PMPCA Antibody [NBP1-89126]

Immunohistochemistry-Paraffin: PMPCA Antibody [NBP1-89126]

Immunohistochemistry-Paraffin: PMPCA Antibody [NBP1-89126] - Staining in human adrenal gland and pancreas tissues using anti-PMPCA antibody. Corresponding PMPCA RNA-seq data are presented for the same tissues.
Western Blot: PMPCA Antibody [NBP1-89126]

Western Blot: PMPCA Antibody [NBP1-89126]

Western Blot: PMPCA Antibody [NBP1-89126] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PMPCA antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western Blot: PMPCA Antibody [NBP1-89126]

Western Blot: PMPCA Antibody [NBP1-89126]

Western Blot: PMPCA Antibody [NBP1-89126] - Analysis using Anti-PMPCA antibody NBP1-89126 (A) shows similar pattern to independent antibody NBP2-57169 (B).

Applications for PMPCA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMPCA

Cleaves presequences (transit peptides) from mitochondrial protein precursors

Alternate Names

Alpha-MPPMGC104197, EC 3.4.24.64, inositol polyphosphate-5-phosphatase, 72 kD, INPP5E, KIAA0123FLJ26258, mitochondrial matrix processing protease, alpha subunit, mitochondrial-processing peptidase subunit alpha, MPPA, P-55, peptidase (mitochondrial processing) alpha

Gene Symbol

PMPCA

Additional PMPCA Products

Product Documents for PMPCA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PMPCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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