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PNMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19877

Novus Biologicals, part of Bio-Techne
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NBP2-19877

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PNMT. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Porcine (89%), Rat (86%), Bovine (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PNMT Antibody

Western Blot: PNMT Antibody [NBP2-19877]

Western Blot: PNMT Antibody [NBP2-19877]

Western Blot: PNMT Antibody [NBP2-19877] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: PNMT Antibody [NBP2-19877]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP2-19877]

Immunohistochemistry-Paraffin: PNMT Antibody [NBP2-19877] - Analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
PNMT Antibody

Western Blot: PNMT Antibody [NBP2-19877] -

Western Blot: PNMT Antibody [NBP2-19877] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (NBP2-19877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for PNMT Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PNMT

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]

Long Name

Phenylethanolamine N-Methyltransferase

Alternate Names

Noradrenaline N-methyltransferase, PENT, PNMTase

Gene Symbol

PNMT

UniProt

Additional PNMT Products

Product Documents for PNMT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PNMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.