PNPO Antibody (OTI1G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45361

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1G9

Concentration

1 mg/ml

View all PNPO Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PNPO Antibody (OTI1G9)

Western Blot: PNPO Antibody (OTI1G9) [NBP2-45361]

Western Blot: PNPO Antibody (OTI1G9) [NBP2-45361]

Western Blot: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO .
Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361]

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361]

Immunohistochemistry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Adenocarcinoma of Human colon tissue.
Flow Cytometry: PNPO Antibody (OTI1G9) [NBP2-45361]

Flow Cytometry: PNPO Antibody (OTI1G9) [NBP2-45361]

Flow Cytometry: PNPO Antibody (OTI1G9) [NBP2-45361] - Analysis of Jurkat cells, using PNPO antibody,(Red), compared to a nonspecific negative control antibody (Blue).
View 12 more images

Applications for PNPO Antibody (OTI1G9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PNPO

Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5).[supplied by OMIM]

Alternate Names

EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase

Gene Symbol

PNPO

Additional PNPO Products

Product Documents for PNPO Antibody (OTI1G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PNPO Antibody (OTI1G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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