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PNPT1 Antibody (4C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43725

Novus Biologicals, part of Bio-Techne
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NBP2-43725

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to human PNPT1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PNPT1 Antibody (4C11)

Western Blot: PNPT1 Antibody (4C11) [NBP2-43725]

Western Blot: PNPT1 Antibody (4C11) [NBP2-43725]

Western Blot: PNPT1 Antibody (4C11) [NBP2-43725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
PNPT1 Antibody (4C11)

Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody (4C11) [NBP2-43725] -

Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody (4C11) [NBP2-43725] - PNPT1 antibody [4C11] detects PNPT1 protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PNPT1 stained by PNPT1 antibody [4C11] (NBP2-43725) diluted at 1:500.
Blue: Fluoroshield with DAPI .
Scale bar= 10um.

Applications for PNPT1 Antibody (4C11)

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH7), 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNPT1

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Alternate Names

DKFZp762K1914, EC 2.7.7, EC 2.7.7.8, mitochondrial, OLD35, old-35, PNPase, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, polyribonucleotide nucleotidyltransferase 1

Gene Symbol

PNPT1

Additional PNPT1 Products

Product Documents for PNPT1 Antibody (4C11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PNPT1 Antibody (4C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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