Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PNUTS Antibodies »

Product Specifications

Immunogen

A synthetic peptide, mapping to a region region between residue 900 and the C-terminus (residue 940) of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31877321).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PNUTS/PPP1R10 Antibody

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605]

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605]

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605] - Detection of Human PNUTS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody NB100-605 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PNUTS/PPP1R10 Antibody [NB100-605]

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605]

Western Blot: PNUTS/PPP1R10 Antibody [NB100-605] - Detection of Human PNUTS on HeLa whole cell lysates usings NB100-605. IP of PNUTS was successfully performed with NB100-603, NB100-604, and another PNUTS polyclonal. IPed PNUTS was blotted using NB100-605

Applications for PNUTS/PPP1R10 Antibody

Application
Recommended Usage

Immunoprecipitation

1-10 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
NB 100-605 may be used for Western blot. Suggested working dilutions: * Western Blot: 1:1,000 - 1:5,000 Immunoprecipitation: 1 to 10 ug/mg lysate Histo/Cytochemistry: not tested. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PNUTS

Protein phosphatase 1 (PP1) is found in the cell nucleus and has been implicated in several aspects of nuclear function. Phosphatase 1 nuclear targeting subunit (PNUTS) is a novel protein that exhibits a discreet nuclear compartmentalization and is colocalized with chromatin at distinct phases during mitosis. The subcellular localization of PP1 and the activity toward substrates involved in many aspects of cell physiology have previously been shown to be regulated by association with noncatalytic targeting subunits. The properties of PNUTS are consistent with its role as a targeting subunit for the regulation of nuclear PP1 function.

Long Name

Phosphatase-1 Nuclear Targeting Subunit

Alternate Names

PPP1R10

Entrez Gene IDs

5514 (Human)

Gene Symbol

PPP1R10

UniProt

Q96QC0 (Human)

Additional PNUTS Products

Product Documents for PNUTS/PPP1R10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PNUTS/PPP1R10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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