PNUTS/PPP1R10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38565

Novus Biologicals, part of Bio-Techne
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NBP2-38565
NBP2-38565-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PNUTS Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PNUTS/PPP1R10 Antibody

Western Blot: PNUTS/PPP1R10 Antibody [NBP2-38565]

Western Blot: PNUTS/PPP1R10 Antibody [NBP2-38565]

Western Blot: PNUTS/PPP1R10 Antibody [NBP2-38565] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunocytochemistry/ Immunofluorescence: PNUTS/PPP1R10 Antibody [NBP2-38565]

Immunocytochemistry/ Immunofluorescence: PNUTS/PPP1R10 Antibody [NBP2-38565]

Immunocytochemistry/Immunofluorescence: PNUTS/PPP1R10 Antibody [NBP2-38565] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
Immunohistochemistry-Paraffin: PNUTS/PPP1R10 Antibody [NBP2-38565]

Immunohistochemistry-Paraffin: PNUTS/PPP1R10 Antibody [NBP2-38565]

Immunohistochemistry-Paraffin: PNUTS/PPP1R10 Antibody [NBP2-38565] - Staining of human skin.
View 5 more images

Applications for PNUTS/PPP1R10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNUTS

Protein phosphatase 1 (PP1) is found in the cell nucleus and has been implicated in several aspects of nuclear function. Phosphatase 1 nuclear targeting subunit (PNUTS) is a novel protein that exhibits a discreet nuclear compartmentalization and is colocalized with chromatin at distinct phases during mitosis. The subcellular localization of PP1 and the activity toward substrates involved in many aspects of cell physiology have previously been shown to be regulated by association with noncatalytic targeting subunits. The properties of PNUTS are consistent with its role as a targeting subunit for the regulation of nuclear PP1 function.

Long Name

Phosphatase-1 Nuclear Targeting Subunit

Alternate Names

PPP1R10

Gene Symbol

PPP1R10

UniProt

Q96QC0 (Human)

Additional PNUTS Products

Product Documents for PNUTS/PPP1R10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PNUTS/PPP1R10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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