POLG2 Antibody (1A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00011232-M01

Novus Biologicals, part of Bio-Techne
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H00011232-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLG2 Antibodies »

Product Specifications

Immunogen

POLG2 (AAH09194, 1 a.a. ~ 485 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRSRVAVRACHKVCRCLLSGFGGRVGAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

Specificity

POLG2 - polymerase (DNA directed), gamma 2, accessory subunit (1A2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for POLG2 Antibody (1A2)

Western Blot: POLG2 Antibody (1A2) [H00011232-M01]

Western Blot: POLG2 Antibody (1A2) [H00011232-M01]

Western Blot: POLG2 Antibody (1A2) [H00011232-M01] - Analysis of POLG2 expression in transfected 293T cell line by POLG2 monoclonal antibody (M01), clone 1A2.Lane 1: POLG2 transfected lysate(54.9 KDa).Lane 2: Non-transfected lysate.

Applications for POLG2 Antibody (1A2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POLG2

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM]

Alternate Names

DNA polymerase gamma accessory 55 kDa subunit, DNA polymerase subunit gamma-2, mitochondrial, EC 2.7.7.7, HP55, Mitochondrial DNA polymerase accessory subunit, mitochondrial DNA polymerase subunit gamma-2, mitochondrial DNA polymerase, accessory subunit, MtPolB, MTPOLBPOLG-BETA, p55, PEOA4, POLB, POLGB, PolG-beta, polymerase (DNA directed), gamma 2, accessory subunit

Entrez Gene IDs

11232 (Human)

Gene Symbol

POLG2

UniProt

AAH09194 (Human)

Additional POLG2 Products

Product Documents for POLG2 Antibody (1A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLG2 Antibody (1A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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