POLG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25059

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-25059
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLG2 Antibodies »

Product Summary for POLG2 Antibody

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein POLG2 using the following amino acid sequence: MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLG2 Antibody

POLG2 Antibody Immunocytochemistry/Immunofluorescence: POLG2 Antibody [NBP3-25059]

Immunocytochemistry/Immunofluorescence: POLG2 Antibody [NBP3-25059]

Staining of human cell line U2OS shows localization to mitochondria.
POLG2 Antibody Western Blot: POLG2 Antibody [NBP3-25059]

Western Blot: POLG2 Antibody [NBP3-25059]

Analysis in human cell line RT-4.

Applications for POLG2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLG2

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM]

Alternate Names

DNA polymerase gamma accessory 55 kDa subunit, DNA polymerase subunit gamma-2, mitochondrial, EC 2.7.7.7, HP55, Mitochondrial DNA polymerase accessory subunit, mitochondrial DNA polymerase subunit gamma-2, mitochondrial DNA polymerase, accessory subunit, MtPolB, MTPOLBPOLG-BETA, p55, PEOA4, POLB, POLGB, PolG-beta, polymerase (DNA directed), gamma 2, accessory subunit

Gene Symbol

POLG2

Additional POLG2 Products

Product Documents for POLG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.