POLR1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-53191

Novus Biologicals, part of Bio-Techne
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NBP1-53191
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all POLR1B Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to POLR1B (polymerase (RNA) I polypeptide B, 128kDa) The peptide sequence was selected from the middle region of POLR1B)(50ug). Peptide sequence SDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHD. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for POLR1B Antibody

Western Blot: POLR1B Antibody [NBP1-53191]

Western Blot: POLR1B Antibody [NBP1-53191]

Western Blot: POLR1B Antibody [NBP1-53191] - Titration: 0.2-1 ug/ml, Positive Control: ACHN cell lysate.

Applications for POLR1B Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR1B

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).

Alternate Names

DNA-directed RNA polymerase I 135 kDa polypeptide, DNA-directed RNA polymerase I 135kDa polypeptide, DNA-directed RNA polymerase I subunit RPA2, EC 2.7.7.6, FLJ10816, FLJ21921, MGC131780, polymerase (RNA) I polypeptide B, 128kDa, RNA polymerase I subunit 2, RPA116, RPA135, RPA2, Rpo1-2

Gene Symbol

POLR1B

UniProt

Q9H9Y6 (Human)

Additional POLR1B Products

Product Documents for POLR1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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