POLR1C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87114

Novus Biologicals, part of Bio-Techne
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NBP1-87114
NBP1-87114-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR1C Antibodies »

Product Summary for POLR1C Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:NNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR1C Antibody

Western Blot: POLR1C Antibody [NBP1-87114]

Western Blot: POLR1C Antibody [NBP1-87114]

Western Blot: POLR1C Antibody [NBP1-87114] - Analysis in control (vector only transfected HEK293T lysate) and POLR1C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]

Immunocytochemistry/ Immunofluorescence: POLR1C Antibody [NBP1-87114]

Immunocytochemistry/Immunofluorescence: POLR1C Antibody [NBP1-87114] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.

Applications for POLR1C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR1C

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft

Alternate Names

AC40, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide, POLR1E, polymerase (RNA) I polypeptide C, 30kDa, RNA polymerase I subunit, RPA39RNA polymerases I and III subunit AC1, RPA40TCS3, RPA5RPAC1DNA-directed RNA polymerases I and III subunit RPAC1, RPC40

Gene Symbol

POLR1C

Additional POLR1C Products

Product Documents for POLR1C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR1C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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