POLR2E Antibody (OTI3B5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73518

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all POLR2E Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2E Antibody (OTI3B5) - Azide and BSA Free

Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]

Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]

Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]

Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]

Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]

Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-POLR2E antibody, and then analyzed by flow cytometry.
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Applications for POLR2E Antibody (OTI3B5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2E

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Gene Symbol

POLR2E

Additional POLR2E Products

Product Documents for POLR2E Antibody (OTI3B5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2E Antibody (OTI3B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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