POLR2E Antibody (S05-8J5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14993

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14993-100ul
NBP3-14993-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-8J5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all POLR2E Antibodies »

Product Summary for POLR2E Antibody (S05-8J5)

Immunogen

A synthetic peptide of human POLR2E (Uniprot#: P19388)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2E Antibody (S05-8J5)

Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993]

Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993]

Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993] - Western blot detection of POLR2E in K562, C6, 3T3, Hela cell lysates using POLR2E Rabbit mAb (1:1000 diluted). Predicted band size: 25kDa. Observed band size: 25kDa.
Immunohistochemistry-Paraffin: POLR2E Antibody (S05-8J5) [NBP3-14993]

Immunohistochemistry-Paraffin: POLR2E Antibody (S05-8J5) [NBP3-14993]

Immunohistochemistry-Paraffin: POLR2E Antibody (S05-8J5) [NBP3-14993] - Immunohistochemistry of POLR2E in paraffin-embedded Human breast cancer tissue using POLR2E Rabbit mAb at dilution 1/20

Applications for POLR2E Antibody (S05-8J5)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2E

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Gene Symbol

POLR2E

Additional POLR2E Products

Product Documents for POLR2E Antibody (S05-8J5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2E Antibody (S05-8J5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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