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POLR2E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15100

Novus Biologicals, part of Bio-Techne
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NBP2-15100

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (98%), Rat (98%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for POLR2E Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2E Antibody

Western Blot: POLR2E Antibody [NBP2-15100]

Western Blot: POLR2E Antibody [NBP2-15100]

Western Blot: POLR2E Antibody [NBP2-15100] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: POLR2E Antibody [NBP2-15100]

Immunocytochemistry/ Immunofluorescence: POLR2E Antibody [NBP2-15100]

Immunocytochemistry/Immunofluorescence: POLR2E Antibody [NBP2-15100] - Paraformaldehyde-fixed A549, using antibody at 1:500 dilution.

Applications for POLR2E Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POLR2E

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Gene Symbol

POLR2E

UniProt

Additional POLR2E Products

Product Documents for POLR2E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR2E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.