POLR2F Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81714

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-81714
NBP1-81714-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR2F Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELII

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR2F Antibody

Western Blot: POLR2F Antibody [NBP1-81714]

Western Blot: POLR2F Antibody [NBP1-81714]

Western Blot: POLR2F Antibody [NBP1-81714] - Analysis in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: POLR2F Antibody [NBP1-81714]

Immunocytochemistry/ Immunofluorescence: POLR2F Antibody [NBP1-81714]

Immunocytochemistry/Immunofluorescence: POLR2F Antibody [NBP1-81714] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: POLR2F Antibody [NBP1-81714]

Immunohistochemistry-Paraffin: POLR2F Antibody [NBP1-81714]

Immunohistochemistry-Paraffin: POLR2F Antibody [NBP1-81714] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.

Applications for POLR2F Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2F

POLR2F encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. [provided by RefSeq]

Alternate Names

DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, HRBP14.4, II, and III subunit RPABC2, polymerase (RNA) II (DNA directed) polypeptide F, RNA Polymerase II subunit 14.4 kD, RNA polymerases I, II, and III subunit ABC2, RPB6, RPB6 homolog

Gene Symbol

POLR2F

Additional POLR2F Products

Product Documents for POLR2F Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLR2F Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.