POLR3D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49530

Novus Biologicals, part of Bio-Techne
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NBP2-49530
NBP2-49530-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR3D Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3D Antibody

Western Blot: POLR3D Antibody [NBP2-49530]

Western Blot: POLR3D Antibody [NBP2-49530]

Western Blot: POLR3D Antibody [NBP2-49530] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: POLR3D Antibody [NBP2-49530]

Immunocytochemistry/ Immunofluorescence: POLR3D Antibody [NBP2-49530]

Immunocytochemistry/Immunofluorescence: POLR3D Antibody [NBP2-49530] - Staining of human cell line SK-MEL-30 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: POLR3D Antibody [NBP2-49530]

Immunohistochemistry-Paraffin: POLR3D Antibody [NBP2-49530]

Immunohistochemistry-Paraffin: POLR3D Antibody [NBP2-49530] - Staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.

Applications for POLR3D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3D

RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. POLR3D include RNA polymerase III subunit C4, RPC4, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III 47 kDa polypeptide, RPC53, protein BN51, TSBN51, and BN51T.

Alternate Names

BN51, BN51 (BHK21) temperature sensitivity complementing, BN51TDNA-directed RNA polymerase III 47 kDa polypeptide, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, polymerase (RNA) III (DNA directed) polypeptide D, 44kDa, Protein BN51, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC4, RPC53 homolog, temperature sensitive complementation, cell cycle specific, tsBN51, TSBN51RPC53

Gene Symbol

POLR3D

Additional POLR3D Products

Product Documents for POLR3D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR3D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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