POLR3E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97859

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all POLR3E Antibodies »

Product Summary for POLR3E Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human POLR3E fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for POLR3E Antibody

Western Blot: POLR3E Antibody [NBP2-97859]

Western Blot: POLR3E Antibody [NBP2-97859]

Western Blot: POLR3E Antibody [NBP2-97859] - Anti-POLR3E rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 80 kDa
Immunoprecipitation: POLR3E Antibody [NBP2-97859]

Immunoprecipitation: POLR3E Antibody [NBP2-97859]

Immunoprecipitation: POLR3E Antibody [NBP2-97859] - POLR3E was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-POLR3E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-POLR3E rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 80 kDa

Applications for POLR3E Antibody

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3E

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway

Alternate Names

DNA-directed RNA polymerase III 80 kDa polypeptide, DNA-directed RNA polymerase III subunit RPC5, FLJ10509, KIAA1452, polymerase (RNA) III (DNA directed) polypeptide E (80kD), RNA polymerase III 80 kDa subunit RPC5, RNA polymerase III subunit C5, RPC5, SIN

Gene Symbol

POLR3E

Additional POLR3E Products

Product Documents for POLR3E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR3E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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