POLR3K Antibody (3F5)
Novus Biologicals, part of Bio-Techne | Catalog # H00051728-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3F5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLR3K (AAH11932, 1 a.a. ~ 108 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD
Reactivity Notes
Human. Other species not tested.
Specificity
POLR3K - polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for POLR3K Antibody (3F5)
![Western Blot: POLR3K Antibody (3F5) [H00051728-M01]](https://resources.bio-techne.com/images/products/POLR3K-Antibody-3F5-Western-Blot-H00051728-M01-img0006.jpg "Western Blot: POLR3K Antibody (3F5) [H00051728-M01]")
Western Blot: POLR3K Antibody (3F5) [H00051728-M01]
Western Blot: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of POLR3K expression in transfected 293T cell line by POLR3K monoclonal antibody (M01), clone 3F5.Lane 1: POLR3K transfected lysate(12.3 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01]](https://resources.bio-techne.com/images/products/POLR3K-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00051728-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01]")
Immunocytochemistry/ Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01]
Immunocytochemistry/Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of monoclonal antibody to POLR3K on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: POLR3K Antibody (3F5) [H00051728-M01] - Detection limit for recombinant GST tagged POLR3K is approximately 0.1ng/ml as a capture antibody.
Applications for POLR3K Antibody (3F5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POLR3K
Alternate Names
C11, DNA-directed RNA polymerase III subunit K, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerases III 12.5 kDa polypeptide, hRPC11, HsC11p, polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa), polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa, RNA polymerase III 12.5 kDa subunit, RNA polymerase III subunit (hRPC11), RNA polymerase III subunit C10, RNA polymerase III subunit C11, RNA polymerase III subunit CII, RPC10, RPC11C11-RNP3, RPC12.5
Entrez Gene IDs
51728 (Human)
Gene Symbol
POLR3K
OMIM
606007 (Human)
UniProt
Additional POLR3K Products
Product Documents for POLR3K Antibody (3F5)
Product Specific Notices for POLR3K Antibody (3F5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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