POM121 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19890

Novus Biologicals, part of Bio-Techne
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NBP2-19890
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POM121 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Localization

Nucleus; nuclear pore complex; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POM121 Antibody

Western Blot: POM121 Antibody [NBP2-19890]

Western Blot: POM121 Antibody [NBP2-19890]

Western Blot: POM121 Antibody [NBP2-19890] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: POM121 Antibody [NBP2-19890]

Immunocytochemistry/ Immunofluorescence: POM121 Antibody [NBP2-19890]

Immunocytochemistry/Immunofluorescence: POM121 Antibody [NBP2-19890] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using POM121 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.
Immunohistochemistry-Paraffin: POM121 Antibody [NBP2-19890]

Immunohistochemistry-Paraffin: POM121 Antibody [NBP2-19890]

Immunohistochemistry-Paraffin: POM121 Antibody [NBP2-19890] - HBL435 xenograft, using POM121 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for POM121 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POM121

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Alternate Names

FLJ41820, MGC3792, nuclear envelope pore membrane protein POM 121, nuclear pore membrane protein 121 kDa, nucleoporin Nup121, NUP121, P145, POM121 membrane glycoprotein, POM121A

Gene Symbol

POM121

UniProt

Q96HA1 (Human)

Additional POM121 Products

Product Documents for POM121 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POM121 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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