POMK/SGK196 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13302

Novus Biologicals, part of Bio-Techne
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NBP2-13302
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POMK/SGK196 Antibodies »

Product Summary for POMK/SGK196 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: TSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POMK/SGK196 Antibody

Western Blot: POMK/SGK196 Antibody [NBP2-13302]

Western Blot: POMK/SGK196 Antibody [NBP2-13302]

Western Blot: FLJ23356 Antibody [NBP2-13302] - Analysis in control (vector only transfected HEK293T lysate) and POMK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: POMK/SGK196 Antibody [NBP2-13302]

Immunocytochemistry/ Immunofluorescence: POMK/SGK196 Antibody [NBP2-13302]

Immunocytochemistry/Immunofluorescence: FLJ23356 Antibody [NBP2-13302] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & cytosol.
Immunohistochemistry-Paraffin: POMK/SGK196 Antibody [NBP2-13302]

Immunohistochemistry-Paraffin: POMK/SGK196 Antibody [NBP2-13302]

Immunohistochemistry-Paraffin: FLJ23356 Antibody [NBP2-13302] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and endothelial cells.

Applications for POMK/SGK196 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POMK/SGK196

Alternate Names

FLJ23356, MGC126597, protein kinase-like protein SgK196, Sugen kinase 196

Gene Symbol

POMK

Additional POMK/SGK196 Products

Product Documents for POMK/SGK196 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POMK/SGK196 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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