POR/Cytochrome P450 Reductase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85357

Novus Biologicals, part of Bio-Techne
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NBP1-85357
NBP1-85357-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POR/Cytochrome P450 Reductase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POR/Cytochrome P450 Reductase Antibody

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human liver and skeletal muscle tissues. Corresponding POR RNA-seq data are presented for the same tissues.
Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [NBP1-85357]

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
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Applications for POR/Cytochrome P450 Reductase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells. P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450. This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.

Long Name

NADPH Cytochrome P450 Reductase

Alternate Names

CPR, CYPOR, Cytochrome P450 Reductase, P450R

Gene Symbol

POR

Additional POR/Cytochrome P450 Reductase Products

Product Documents for POR/Cytochrome P450 Reductase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POR/Cytochrome P450 Reductase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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