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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # OCT2/7073R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human POU2F2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)

Localization

Nucleus

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13743).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

OCT2 Antibody (OCT2/7073R) - Azide and BSA Free

OCT2 Antibody (OCT2/7073R)

Formalin-fixed, paraffin-embedded human tonsil stained with OCT2 antibody (OCT2/7073R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
OCT2 Antibody (OCT2/7073R) - Azide and BSA Free

OCT2 Antibody (OCT2/7073R)

Formalin-fixed, paraffin-embedded human tonsil stained with OCT2 antibody (OCT2/7073R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: POU2F2

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Alternate Names

homeobox protein, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, OCT2, Octamer-binding protein 2, Octamer-binding transcription factor 2, OTF2, OTF-2, POU class 2 homeobox 2, POU domain, class 2, transcription factor 2

Gene Symbol

POU2F2

Additional POU2F2 Products

Product Documents for POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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