POU3F2/OCT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24658

Novus Biologicals, part of Bio-Techne
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NBP2-24658
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Equine, Primate, Rhesus Macaque

Predicted:

Chimpanzee (100%), Monkey (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all POU3F2 Antibodies »

Product Summary for POU3F2/OCT7 Antibody

Immunogen

A portion of amino acids 240 to 290 of human POU3F2 was used as immunogen for the antibody.

Reactivity Notes

The amino acid sequence used as immunogen is 87% homologous in Equine, 81% homologous in Bovine and Porcine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POU3F2/OCT7 Antibody

Western Blot: POU3F2/OCT7 Antibody [NBP2-24658]

Western Blot: POU3F2/OCT7 Antibody [NBP2-24658]

Western Blot: POU3F2/OCT7 Antibody [NBP2-24658] - Analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-24658]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-24658]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-24658] - Human small intestine stained with POU3F2 antibody at 10 ug/ml.

Applications for POU3F2/OCT7 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

2-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POU3F2

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Gene Symbol

POU3F2

UniProt

P20265

Additional POU3F2 Products

Product Documents for POU3F2/OCT7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for POU3F2/OCT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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