POU3F3 Antibody (CL15308) [Unconjugated]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43660

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-43660-100ul
NBP3-43660-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL15308

Concentration

1 mg/ml

View all POU3F3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Synthetic Peptide of P20264. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for POU3F3 Antibody (CL15308) [Unconjugated]

POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Staining of human cerebellum shows strong nuclear positivity in granular layer and molecular layer cells.
POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]

Staining of human rectum shows no positivity in glandular cells as expected.
View 6 more images

Applications for POU3F3 Antibody (CL15308) [Unconjugated]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000-1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU3F3

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Alternate Names

brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3

Gene Symbol

POU3F3

Additional POU3F3 Products

Product Documents for POU3F3 Antibody (CL15308) [Unconjugated]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POU3F3 Antibody (CL15308) [Unconjugated]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.