POU3F3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49872
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Predicted:
Human (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HMLSHAHQWVTAL corresponding to internal region according to NP_006227.1.
Epitope
HMLSHAHQWVTAL - internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for POU3F3 Antibody
![Western Blot: POU3F3 Antibody [NBP1-49872]](https://resources.bio-techne.com/images/products/POU3F3-Antibody-Western-Blot-NBP1-49872-img0001.jpg "Western Blot: POU3F3 Antibody [NBP1-49872]")
Western Blot: POU3F3 Antibody [NBP1-49872]
Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872]](https://resources.bio-techne.com/images/products/POU3F3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49872-img0002.jpg "Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872]")
Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872]
Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Flow Cytometry: POU3F3 Antibody [NBP1-49872]](https://resources.bio-techne.com/images/products/POU3F3-Antibody-Flow-Cytometry-NBP1-49872-img0003.jpg "Flow Cytometry: POU3F3 Antibody [NBP1-49872]")
Flow Cytometry: POU3F3 Antibody [NBP1-49872]
Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for POU3F3 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:4000
Western Blot
1 - 3 ug/mL
Application Notes
IHC-Fr reactivity reported in (PMID: 25273085). IHC reactivity reported in (PMID: 25224226).
Reviewed Applications
Read 1 review rated 5 using NBP1-49872 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POU3F3
Alternate Names
brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3
Gene Symbol
POU3F3
UniProt
Additional POU3F3 Products
Product Documents for POU3F3 Antibody
Product Specific Notices for POU3F3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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