POU6F1 Antibody (6H1)
Novus Biologicals, part of Bio-Techne | Catalog # H00005463-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 6H1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POU6F1 (NP_002693, 193 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITPKSAQKLKPVLEKWLNEAELRNQEGQQNLMEFVGGEPSKKRKRRTSFTPQAIEALNAYFEKNPLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKLNVFQIP
Specificity
POU6F1 - POU domain, class 6, transcription factor 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for POU6F1 Antibody (6H1)
![Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]](https://resources.bio-techne.com/images/products/POU6F1-Antibody-6H1-Western-Blot-H00005463-M01-img0008.jpg "Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]")
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - Analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody (M01), clone 6H1.Lane 1: POU6F1 transfected lysate(32.6 KDa).Lane 2: Non-transfected lysate.![Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]](https://resources.bio-techne.com/images/products/POU6F1-Antibody-6H1-Western-Blot-H00005463-M01-img0005.jpg "Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]")
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - Analysis of POU6F1 over-expressed 293 cell line, cotransfected with POU6F1 Validated Chimera RNAi ( Cat # H00005463-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POU6F1 monoclonal antibody (M01), clone 6H1 (Cat # H00005463-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]](https://resources.bio-techne.com/images/products/POU6F1-Antibody-6H1-Western-Blot-H00005463-M01-img0007.jpg "Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]")
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01]
Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - POU6F1 monoclonal antibody (M01), clone 6H1 Analysis of POU6F1 expression in Jurkat.Applications for POU6F1 Antibody (6H1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POU6F1
Alternate Names
Brain-specific homeobox/POU domain protein 5, Brn-5, BRN5brain-5, mPOU homeobox protein, MPOUbrn-5, POU class 6 homeobox 1, POU domain, class 6, transcription factor 1, TCFB1Brain-5
Entrez Gene IDs
5463 (Human)
Gene Symbol
POU6F1
UniProt
Additional POU6F1 Products
Product Documents for POU6F1 Antibody (6H1)
Product Specific Notices for POU6F1 Antibody (6H1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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