PP2C alpha/PPM1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98253
![Western Blot: PP2C alpha/PPM1A Antibody [NBP2-98253]](https://resources.bio-techne.com/images/products/PP2C-alpha-PPM1A-Antibody-Western-Blot-NBP2-98253-img0004.jpg "Western Blot: PP2C alpha/PPM1A Antibody [NBP2-98253]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Mouse PP2C alpha/PPM1A (Accession#: NP_032936.1; Met 1-Trp 382)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PP2C alpha/PPM1A Antibody
Western Blot: PP2C alpha/PPM1A Antibody [NBP2-98253]
Western Blot: PP2C alpha/PPM1A Antibody [NBP2-98253] - Anti-PP2C alpha/PPM1A rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 40 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: PP2C alpha/PPM1A Antibody [NBP2-98253]](https://resources.bio-techne.com/images/products/PP2C-alpha-PPM1A-Antibody-Immunoprecipitation-NBP2-98253-img0003.jpg "Immunoprecipitation: PP2C alpha/PPM1A Antibody [NBP2-98253]")
Immunoprecipitation: PP2C alpha/PPM1A Antibody [NBP2-98253]
Immunoprecipitation: PP2C alpha/PPM1A Antibody [NBP2-98253] - Mouse PP2C alpha/PPM1A was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Mouse PP2C alpha/PPM1A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse PP2C alpha/PPM1A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDaApplications for PP2C alpha/PPM1A Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP2C alpha/PPM1A
Long Name
Protein Phosphatase 2C alpha
Alternate Names
MMPa-2, MPPa-1, Pp2c1, PPM1A
Gene Symbol
PPM1A
Additional PP2C alpha/PPM1A Products
Product Documents for PP2C alpha/PPM1A Antibody
Product Specific Notices for PP2C alpha/PPM1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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