Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # k1G6

Format

BSA Free

Concentration

1.0 mg/ml

View all PP2C gamma Antibodies »

Product Specifications

Immunogen

Recombinant human PP2C gamma/PPM1G (317-546aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]

Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]

Western Blot: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Analysis in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Applications for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

UniProt

NP_002698 (Human)

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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