Skip to main content

PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04337

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # k1G6

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Immunogen

Recombinant human PP2C gamma/PPM1G (317-546aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]

Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]

Western Blot: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Analysis in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Applications for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...