PP2C gamma/PPM1G Antibody (OTI1C12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03463
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1C12
Concentration
1 mg/ml
Product Summary for PP2C gamma/PPM1G Antibody (OTI1C12)
Immunogen
Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
59.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PP2C gamma/PPM1G Antibody (OTI1C12)
Western Blot: PPM1G Antibody (1C12) [NBP2-03463] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
![Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]](https://resources.bio-techne.com/images/products/PPM1G-Antibody-1C12-Immunocytochemistry-Immunofluorescence-NBP2-03463-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]")
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]
Immunocytochemistry/Immunofluorescence: PPM1G Antibody (1C12) [NBP2-03463] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G.![Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]](https://resources.bio-techne.com/images/products/PPM1G-Antibody-1C12-Western-Blot-NBP2-03463-img0001.jpg "Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]")
Western Blot: PP2C gamma/PPM1G Antibody (OTI1C12) [NBP2-03463]
Western Blot: PPM1G Antibody (1C12) [NBP2-03463] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G.Applications for PP2C gamma/PPM1G Antibody (OTI1C12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PP2C gamma
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Entrez Gene IDs
5496 (Human)
Gene Symbol
PPM1G
Additional PP2C gamma Products
Product Documents for PP2C gamma/PPM1G Antibody (OTI1C12)
Product Specific Notices for PP2C gamma/PPM1G Antibody (OTI1C12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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