PP2C gamma/PPM1G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99758

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PP2C gamma Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human PP2C gamma/PPM1G (Accession#: O15355; Met 317-Asp 546)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PP2C gamma/PPM1G Antibody

Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758]

Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758]

Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758] - Anti-PP2C gamma/PPM1G rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 292T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 80 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758]

Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758]

Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758] - PP2C gamma/PPM1G was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-PP2C gamma/PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PP2C gamma/PPM1G rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa

Applications for PP2C gamma/PPM1G Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PP2C gamma/PPM1G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.