PP4/PPP4C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51947

Novus Biologicals, part of Bio-Techne
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NBP1-51947
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Canine (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PP4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PQETRGIPSKKP corresponding to C-Terminus according to NP_002711.1.

Epitope

C-PQETRGIPSKKP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PP4/PPP4C Antibody

Western Blot: PP4/PPP4C Antibody [NBP1-51947]

Western Blot: PP4/PPP4C Antibody [NBP1-51947]

Western Blot: PPP4C Antibody [NBP1-51947] - Analysis of Rat Spleen (A) and Lung (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP1-51947]

Immunohistochemistry-Paraffin: PP4/PPP4C Antibody [NBP1-51947]

Immunohistochemistry-Paraffin: PPP4C Antibody [NBP1-51947] - (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: PP4/PPP4C Antibody [NBP1-51947]

Western Blot: PP4/PPP4C Antibody [NBP1-51947]

Western Blot: PPP4C Antibody [NBP1-51947] - Western blot analysis of PPP4C in HEK293 lysate (35ug protein in RIPA buffer) using NBP1-51947 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PP4/PPP4C Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in HEK293, A431 and HeLa lysates and in rat spleen and lung lysates (calculated MW of 35.1 kDa band according to NP_002711.1). IHC-P: Human prostate shows nuclear staining of secretory cells in the gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PP4

Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.

Long Name

Protein Phosphatase 4

Alternate Names

PP4C, PPH3, PPP4C, PPX

Entrez Gene IDs

5531 (Human); 171366 (Rat)

Gene Symbol

PPP4C

UniProt

NP_002711.1 (Human)

Additional PP4 Products

Product Documents for PP4/PPP4C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PP4/PPP4C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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