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PP4/PPP4C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19912

Novus Biologicals, part of Bio-Techne
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NBP2-19912

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP4/PPP4C. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus; Centrosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PP4/PPP4C Antibody

Western Blot: PP4/PPP4C Antibody [NBP2-19912]

Western Blot: PP4/PPP4C Antibody [NBP2-19912]

Western Blot: PPP4C Antibody [NBP2-19912] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: PP4/PPP4C Antibody [NBP2-19912]

Immunocytochemistry/ Immunofluorescence: PP4/PPP4C Antibody [NBP2-19912]

Immunocytochemistry/Immunofluorescence: PPP4C Antibody [NBP2-19912] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: PP4/PPP4C Antibody [NBP2-19912]

Western Blot: PP4/PPP4C Antibody [NBP2-19912]

Western Blot: PPP4C Antibody [NBP2-19912] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:500.

Applications for PP4/PPP4C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PP4

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase.

Long Name

Protein Phosphatase 4

Alternate Names

PP4C, PPH3, PPP4C, PPX

Gene Symbol

PPP4C

UniProt

Additional PP4 Products

Product Documents for PP4/PPP4C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP4/PPP4C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.