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PP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40555

Novus Biologicals, part of Bio-Techne
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NB110-40555

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Protein Phosphatase 5, Catalytic Subunit using the numbering given in entry NP_006238.1 (GeneID 5536).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP5 Antibody

Western Blot: PP5 Antibody [NB110-40555]

Western Blot: PP5 Antibody [NB110-40555]

Western Blot: PP5 Antibody [NB110-40555] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB110-40555 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry: PP5 Antibody [NB110-40555]

Immunohistochemistry: PP5 Antibody [NB110-40555]

Immunohistochemistry: PP5 Antibody [NB110-40555] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PPP5C used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PP5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 -1:1000

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PP5

It may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, it dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Long Name

Protein Phosphatase 5, Catalytic Subunit

Alternate Names

EC 3.1.3.16, PPP5, PPP5C, PPT

Entrez Gene IDs

5536 (Human); 19060 (Mouse)

Gene Symbol

PPP5C

Additional PP5 Products

Product Documents for PP5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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