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PP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87240

Novus Biologicals, part of Bio-Techne
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NBP1-87240

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP5 Antibody

Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240]

Western Blot: PP5 Antibody [NBP1-87240] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: PP5 Antibody [NBP1-87240]

Immunocytochemistry/ Immunofluorescence: PP5 Antibody [NBP1-87240]

Immunocytochemistry/Immunofluorescence: PP5 Antibody [NBP1-87240] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240]

Immunohistochemistry-Paraffin: PP5 Antibody [NBP1-87240] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Applications for PP5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP5

It may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, it dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Long Name

Protein Phosphatase 5, Catalytic Subunit

Alternate Names

EC 3.1.3.16, PPP5, PPP5C, PPT

Gene Symbol

PPP5C

Additional PP5 Products

Product Documents for PP5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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