PPAN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29745

Novus Biologicals, part of Bio-Techne
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NBP3-29745
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PPAN Antibodies »

Product Specifications

Immunogen

Between 375 and 425

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAN Antibody

PPAN Antibody

Immunoprecipitation:PPAN AntibodyNBP3-29745] -

Immunoprecipitation:PPAN AntibodyNBP3-29745] -Detection of human PPAN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAN antibody NBP3-29745 (lot NBP3-29745-1) used for IP at 6 µg per reaction. PPAN was also immunoprecipitated by rabbit anti-PPAN antibody BL17358. For blotting immunoprecipitated PPAN, NBP3-29745 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PPAN Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PPAN

PPAN is encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. This gene was found to cotranscript with P2RY11/P2Y(11), an immediate downstream gene on the chromosome that encodes a ATP receptor. The chimeric transcripts of this gene and P2RY11 were found to be ubiquitously present and regulated during granulocytic differentiation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

Brix domain-containing protein 3, homolog of S. cerevisiae SSF1, MGC14226, MGC45852, peter pan (Drosophila) homolog, Peter Pan homolog, peter pan homolog (Drosophila), second-step splicing factor 1, SSF, Ssf-1, SSF1BXDC3SSF-1, SSF2, suppressor of sterile four 1, suppressor of SWI4 1 homolog

Gene Symbol

PPAN

Additional PPAN Products

Product Documents for PPAN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPAN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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