PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-537

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Rabbit

Cited:

Human, Mouse, Rat, Bovine, Rabbit

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3B6/PPAR

Format

BSA Free

Concentration

1 mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Purified recombinant PPAR alpha protein.

Localization

Nuclear

Specificity

PPAR alpha (3B6/PPAR)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR)BSA Free [NB300-537]

Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR)BSA Free [NB300-537]

Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537]

Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications for PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1 ug / 10^6 cells

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

2 - 5 ug/ml
Application Notes
May be useful in ChIP. Rabbit-IP reported in publication, see Sumanasekera WK et al. Rat-IHC reported in publication, see Crespillo A et al.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-537 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

5465 (Human); 19013 (Mouse); 25747 (Rat)

Gene Symbol

PPARA

UniProt

Q07869

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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