Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8)Azide and BSA Free [NBP2-73567]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8)Azide and BSA Free [NBP2-73567]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free [NBP2-73567] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAR alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAR alpha.
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free [NBP2-73567]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free [NBP2-73567]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free [NBP2-73567] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPAR alpha.
Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8)Azide and BSA Free [NBP2-73567]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8)Azide and BSA Free [NBP2-73567]

Western Blot: PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free [NBP2-73567] - Analysis of PPAR alpha in HeLa cell lysate (25ug) using anti-PPAR alpha antibody. Image from verified customer review.
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Applications for PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Gene Symbol

PPARA

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPAR alpha/NR1C1 Antibody (OTI1E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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