PPAR alpha/NR1C1 Antibody - N-terminal

Western Blot: PPAR alpha/NR1C1 AntibodyN-terminal [NB600-636]

Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.

ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.

PPAR alpha/NR1C1 Antibody - N-terminal

Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.

PPAR alpha/NR1C1 Antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) showing PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).

PPAR alpha/NR1C1 Antibody - N-terminal

Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.

PPAR alpha/NR1C1 Antibody - N-terminal

Western Blot of Rabbit anti-PPAR Alpha (N-terminal specific) antibody. Marker: Opal Pre-stained ladder

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] -

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.