PPIL4 Antibody (1C10)
Novus Biologicals, part of Bio-Techne | Catalog # H00085313-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1C10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPIL4 (NP_624311, 395 a.a. ~ 466 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKEDEDYMPIKNTNQDIYREMGFGHYEEEESCWEKQKSEKRDRTQNRSRSRSRERDGHYSNSHKSKYQTDLY
Reactivity Notes
Human. Other species not tested.
Specificity
PPIL4 - peptidylprolyl isomerase (cyclophilin)-like 4
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for PPIL4 Antibody (1C10)
Western Blot: PPIL4 Antibody (1C10) [H00085313-M01]
Western Blot: PPIL4 Antibody (1C10) [H00085313-M01] - PPIL4 monoclonal antibody (M01), clone 1C10 Analysis of PPIL4 expression in Hela S3 NE.Immunocytochemistry/ Immunofluorescence: PPIL4 Antibody (1C10) [H00085313-M01]
Immunocytochemistry/Immunofluorescence: PPIL4 Antibody (1C10) [H00085313-M01] - Analysis of monoclonal antibody to PPIL4 on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: PPIL4 Antibody (1C10) [H00085313-M01]
Western Blot: PPIL4 Antibody (1C10) [H00085313-M01] - Analysis of PPIL4 over-expressed 293 cell line, cotransfected with PPIL4 Validated Chimera RNAi ( Cat # H00085313-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPIL4 monoclonal antibody (M01), clone 1C10 (Cat # H00085313-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for PPIL4 Antibody (1C10)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PPIL4
Alternate Names
Cyclophilin-like protein PPIL4, cyclophilin-type peptidyl-prolyl cis-trans isomerase, EC 5.2.1.8, HDCME13P, peptidyl-prolyl cis-trans isomerase-like 4, peptidylprolyl isomerase (cyclophilin)-like 4, PPIase, Rotamase PPIL4, serologically defined breast cancer antigen NY-BR-18
Entrez Gene IDs
85313 (Human)
Gene Symbol
PPIL4
OMIM
607609 (Human)
UniProt
Additional PPIL4 Products
Product Documents for PPIL4 Antibody (1C10)
Product Specific Notices for PPIL4 Antibody (1C10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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