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PPIL4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85921

Novus Biologicals, part of Bio-Techne
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NBP1-85921
NBP1-85921-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KVPRIKHKKKGTVSMVNNGSDQHGSQFLITTGENLDYLDGVHTVFGEVTEGMDIIKKINETFVDKDFVPYQDIRINHTVILDDPFDDPPDLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPIL4 Antibody

Western Blot: PPIL4 Antibody [NBP1-85921]

Western Blot: PPIL4 Antibody [NBP1-85921]

Western Blot: PPIL4 Antibody [NBP1-85921] - Analysis using Anti-PPIL4 antibody NBP1-85921 (A) shows similar pattern to independent antibody NBP2-55080 (B).
Immunocytochemistry/ Immunofluorescence: PPIL4 Antibody [NBP1-85921]

Immunocytochemistry/ Immunofluorescence: PPIL4 Antibody [NBP1-85921]

Immunocytochemistry/Immunofluorescence: PPIL4 Antibody [NBP1-85921] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: PPIL4 Antibody [NBP1-85921]

Immunohistochemistry-Paraffin: PPIL4 Antibody [NBP1-85921]

Immunohistochemistry-Paraffin: PPIL4 Antibody [NBP1-85921] - Staining of human urinary bladder shows nuclear positivity in urothelial cells.

Applications for PPIL4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPIL4

PPIL4 is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions.

Alternate Names

Cyclophilin-like protein PPIL4, cyclophilin-type peptidyl-prolyl cis-trans isomerase, EC 5.2.1.8, HDCME13P, peptidyl-prolyl cis-trans isomerase-like 4, peptidylprolyl isomerase (cyclophilin)-like 4, PPIase, Rotamase PPIL4, serologically defined breast cancer antigen NY-BR-18

Gene Symbol

PPIL4

Additional PPIL4 Products

Product Documents for PPIL4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPIL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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