PPME1 Antibody (OTI7F12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02240
![Flow Cytometry: PPME1 Antibody (OTI7F12) [NBP2-02240]](https://resources.bio-techne.com/images/products/PPME1-Antibody-7F12-Flow-Cytometry-NBP2-02240-img0002.jpg "Flow Cytometry: PPME1 Antibody (OTI7F12) [NBP2-02240]")
![Flow Cytometry: PPME1 Antibody (OTI7F12) [NBP2-02240]](https://resources.bio-techne.com/images/products/PPME1-Antibody-7F12-Flow-Cytometry-NBP2-02240-img0001.jpg "Flow Cytometry: PPME1 Antibody (OTI7F12) [NBP2-02240]")
![Western Blot: PPME1 Antibody (OTI7F12) [NBP2-02240]](https://resources.bio-techne.com/images/products/PPME1-Antibody-7F12-Western-Blot-NBP2-02240-img0003.jpg "Western Blot: PPME1 Antibody (OTI7F12) [NBP2-02240]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI7F12
Concentration
0.96 mg/ml
Product Summary for PPME1 Antibody (OTI7F12)
Immunogen
Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PPME1 Antibody (OTI7F12)
Western Blot: PPME1 Antibody (OTI7F12) [NBP2-02240]
Western Blot: PPME1 Antibody (7F12) [NBP2-02240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
Immunocytochemistry/Immunofluorescence: PPME1 Antibody (7F12) [NBP2-02240] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.
![Immunohistochemistry-Paraffin: PPME1 Antibody (OTI7F12) [NBP2-02240]](https://resources.bio-techne.com/images/products/PPME1-Antibody-7F12-Immunohistochemistry-Paraffin-NBP2-02240-img0004.jpg "Immunohistochemistry-Paraffin: PPME1 Antibody (OTI7F12) [NBP2-02240]")
Immunohistochemistry-Paraffin: PPME1 Antibody (OTI7F12) [NBP2-02240]
Immunohistochemistry-Paraffin: PPME1 Antibody (7F12) [NBP2-02240] - Staining of paraffin-embedded Kidney tissue using anti-PPME1mouse monoclonal antibody.Applications for PPME1 Antibody (OTI7F12)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.96 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PPME1
Alternate Names
EC 3.1.1, EC 3.1.1.-, FLJ22226, PME-1PME1, protein phosphatase methylesterase 1, protein phosphatase methylesterase-1
Entrez Gene IDs
51400 (Human)
Gene Symbol
PPME1
Additional PPME1 Products
Product Documents for PPME1 Antibody (OTI7F12)
Product Specific Notices for PPME1 Antibody (OTI7F12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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