PPP2R1B Antibody (PSH02-92)

Immunohistochemistry: PPP2R1B Antibody (PSH02-92) [NBP3-32845]

Immunohistochemistry: PPP2R1B Antibody (PSH02-92) [NBP3-32845] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (NBP3-32845) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32845) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: PPP2R1B Antibody (PSH02-92) [NBP3-32845]

Western Blot: PPP2R1B Antibody (PSH02-92) [NBP3-32845] - Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (NBP3-32845) at 1/2,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.