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PPP2R1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87251

Novus Biologicals, part of Bio-Techne
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NBP1-87251
NBP1-87251-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPP2R1B Antibody

Western Blot: PPP2R1B Antibody [NBP1-87251]

Western Blot: PPP2R1B Antibody [NBP1-87251]

Western Blot: PPP2R1B Antibody [NBP1-87251] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PPP2R1B Antibody [NBP1-87251]

Immunocytochemistry/ Immunofluorescence: PPP2R1B Antibody [NBP1-87251]

Immunocytochemistry/Immunofluorescence: PPP2R1B Antibody [NBP1-87251] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251]

Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251]

Immunohistochemistry-Paraffin: PPP2R1B Antibody [NBP1-87251] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for PPP2R1B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPP2R1B

The PPP2R1B gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric co

Alternate Names

MGC26454, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PP2A, subunit A, PR65-beta isoform, PP2A, subunit A, R1-beta isoform, PP2A-Abeta, PR65B, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform, protein phosphatase 2, regulatory subunit A, beta, protein phosphatase 2, structural/regulatory subunit A, beta, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A betaisoform

Gene Symbol

PPP2R1B

Additional PPP2R1B Products

Product Documents for PPP2R1B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPP2R1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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