PPP2R4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51951

Novus Biologicals, part of Bio-Techne
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NBP1-51951
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

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Product Specifications

Immunogen

Peptide with sequence C-GSLLPIHPVTSG corresponding to C-Terminus according to NP_066954.2, NP_821070.1.

Epitope

C-GSLLPIHPVTSG

Specificity

This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PPP2R4 Antibody

Western Blot: PPP2R4 Antibody [NBP1-51951]

Western Blot: PPP2R4 Antibody [NBP1-51951]

Western Blot: PPP2R4 Antibody [NBP1-51951] - Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951]

Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PPP2R4 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human, mouse and rat liver lysates and in HepG2 lysates (calculated MW of 36.8 kDa band according to NP_066954.2).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R4

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Entrez Gene IDs

5524 (Human); 110854 (Mouse); 362102 (Rat)

Gene Symbol

PTPA

UniProt

NP_821068.1 (Human)

Additional PPP2R4 Products

Product Documents for PPP2R4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPP2R4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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