PPP4R2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2877
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 370 and the C-terminus (residue 417) of human Protein Phosphatase 4, Regulatory Subunit 2 using the numbering given in entry NP_777567.1 (GeneID 151987).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PPP4R2 Antibody
![Western Blot: PPP4R2 Antibody [NB100-2877]](https://resources.bio-techne.com/images/products/PPP4R2-Antibody-Western-Blot-NB100-2877-img0008.jpg "Western Blot: PPP4R2 Antibody [NB100-2877]")
Western Blot: PPP4R2 Antibody [NB100-2877]
Western Blot: PPP4R2 Antibody [NB100-2877] - Detection of Human and Mouse PPP4R2 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877]](https://resources.bio-techne.com/images/products/PPP4R2-Antibody-Immunohistochemistry-NB100-2877-img0005.jpg "Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877]")
Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877]
Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PPP4R2 used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunoprecipitation: PPP4R2 Antibody [NB100-2877]](https://resources.bio-techne.com/images/products/PPP4R2-Antibody-Immunoprecipitation-NB100-2877-img0009.jpg "Immunoprecipitation: PPP4R2 Antibody [NB100-2877]")
Immunoprecipitation: PPP4R2 Antibody [NB100-2877]
Immunoprecipitation: PPP4R2 Antibody [NB100-2877] - Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 (lotNB100-2877-2) used for IP at 6 ug per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2877-1). For blotting immunoprecipitated PPP4R2 ,NB100-2877 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for PPP4R2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100- 1:500
Immunohistochemistry-Paraffin
1:100- 1:500
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PPP4R2
Alternate Names
MGC131930, PP4R2, protein phosphatase 4, regulatory subunit 2, serine/threonine-protein phosphatase 4 regulatory subunit 2
Gene Symbol
PPP4R2
UniProt
Additional PPP4R2 Products
Product Documents for PPP4R2 Antibody
Product Specific Notices for PPP4R2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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