PPP4R2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99605
![Western Blot: PPP4R2 Antibody [NBP2-99605]](https://resources.bio-techne.com/images/products/PPP4R2-Antibody-Western-Blot-NBP2-99605-img0003.jpg "Western Blot: PPP4R2 Antibody [NBP2-99605]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PPP4R2 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PPP4R2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PPP4R2 Antibody
Western Blot: PPP4R2 Antibody [NBP2-99605]
Western Blot: PPP4R2 Antibody [NBP2-99605] - Anti-PPP4R2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa![Immunocytochemistry/ Immunofluorescence: PPP4R2 Antibody [NBP2-99605]](https://resources.bio-techne.com/images/products/PPP4R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99605-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PPP4R2 Antibody [NBP2-99605]")
Immunocytochemistry/ Immunofluorescence: PPP4R2 Antibody [NBP2-99605]
Immunocytochemistry/Immunofluorescence: PPP4R2 Antibody [NBP2-99605] - Immunofluorescence staining of PPP4R2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PPP4R2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .Applications for PPP4R2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PPP4R2
Alternate Names
MGC131930, PP4R2, protein phosphatase 4, regulatory subunit 2, serine/threonine-protein phosphatase 4 regulatory subunit 2
Gene Symbol
PPP4R2
Additional PPP4R2 Products
Product Specific Notices for PPP4R2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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