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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 150 and 200 of human Polyglutamine Binding Protein 1 using the numbering given in entry NP_005701.1 (GeneID 10084).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PQBP1 Antibody

Western Blot: PQBP1 Antibody [NBP1-47270]

Western Blot: PQBP1 Antibody [NBP1-47270]

Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry: PQBP1 Antibody [NBP1-47270]

Immunohistochemistry: PQBP1 Antibody [NBP1-47270]

Immunohistochemistry: PQBP1 Antibody [NBP1-47270] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:1,000 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for PQBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate.

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PQBP1

PQBP1 is a nuclear polyglutamine-binding protein that contains a WW domain (Waragai et al., 1999 [PubMed 10332029]).[supplied by OMIM]

Alternate Names

38 kDa nuclear protein containing a WW domain, MRX55, MRXS3, MRXS8, Npw38, NPW38mental retardation, X-linked 55, nuclear protein containing WW domain 38 kD, polyglutamine binding protein 1, Polyglutamine tract-binding protein 1, polyglutamine-binding protein 1, PQBP-1, RENS1, SHS, Sutherland-Haan X-linked mental retardation syndrome

Entrez Gene IDs

10084 (Human)

Gene Symbol

PQBP1

Additional PQBP1 Products

Product Documents for PQBP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PQBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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