PQBP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47271
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 215 and 265 of human Polyglutamine Binding Protein 1 using the numbering given in entry NP_005701.1 (GeneID 10084).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PQBP1 Antibody
![Western Blot: PQBP1 Antibody [NBP1-47271]](https://resources.bio-techne.com/images/products/PQBP1-Antibody-Western-Blot-NBP1-47271-img0002.jpg "Western Blot: PQBP1 Antibody [NBP1-47271]")
Western Blot: PQBP1 Antibody [NBP1-47271]
Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).![Immunohistochemistry: PQBP1 Antibody [NBP1-47271]](https://resources.bio-techne.com/images/products/PQBP1-Antibody-Immunohistochemistry-NBP1-47271-img0003.jpg "Immunohistochemistry: PQBP1 Antibody [NBP1-47271]")
Immunohistochemistry: PQBP1 Antibody [NBP1-47271]
Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory KitApplications for PQBP1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PQBP1
Alternate Names
38 kDa nuclear protein containing a WW domain, MRX55, MRXS3, MRXS8, Npw38, NPW38mental retardation, X-linked 55, nuclear protein containing WW domain 38 kD, polyglutamine binding protein 1, Polyglutamine tract-binding protein 1, polyglutamine-binding protein 1, PQBP-1, RENS1, SHS, Sutherland-Haan X-linked mental retardation syndrome
Entrez Gene IDs
10084 (Human)
Gene Symbol
PQBP1
Additional PQBP1 Products
Product Documents for PQBP1 Antibody
Product Specific Notices for PQBP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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