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PQBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99606

Novus Biologicals, part of Bio-Techne
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NBP2-99606-100ul
NBP2-99606-50ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for PQBP1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PQBP1.

Reactivity Notes

Gorilla (100%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PQBP1 Antibody

Western Blot: PQBP1 Antibody [NBP2-99606]

Western Blot: PQBP1 Antibody [NBP2-99606]

Western Blot: PQBP1 Antibody [NBP2-99606] - Anti-PQBP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 38 kDa
Immunocytochemistry/ Immunofluorescence: PQBP1 Antibody [NBP2-99606]

Immunocytochemistry/ Immunofluorescence: PQBP1 Antibody [NBP2-99606]

Immunocytochemistry/Immunofluorescence: PQBP1 Antibody [NBP2-99606] - Immunofluorescence staining of PQBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PQBP1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

Applications for PQBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PQBP1

PQBP1, also known as Polyglutamine-binding protein 1, is a nuclear polyglutamine-binding protein that may play a role in apoptosis and DNA replication. Ten isoforms of PQBP1 have been identified which are produced by alternative splicing. The canonical sequence, referred to as PQBP-1, is 265 amino acids long and approximately 30kdA. PQBP1 may be implicated in an x-linked mental retardation syndrome called Renpenning syndrome 1 (RENS1), as well as neurodegenerative diseases, coloboma, spastic diplegia, ataxia and neuronitis. PQBP1 has also been shown to interact with C14orf1, MED31, WBP11, CLTB, and SF3A2.

Alternate Names

38 kDa nuclear protein containing a WW domain, MRX55, MRXS3, MRXS8, Npw38, NPW38mental retardation, X-linked 55, nuclear protein containing WW domain 38 kD, polyglutamine binding protein 1, Polyglutamine tract-binding protein 1, polyglutamine-binding protein 1, PQBP-1, RENS1, SHS, Sutherland-Haan X-linked mental retardation syndrome

Gene Symbol

PQBP1

Additional PQBP1 Products

Product Documents for PQBP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PQBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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