PRAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00064425-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRAF1 Antibodies »

Product Specifications

Immunogen

POLR1E (NP_071935.1, 1 a.a. - 419 a.a.) full-length human protein. MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIEAFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDFQIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT

Specificity

POLR1E - polymerase (RNA) I polypeptide E, 53kDa,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for PRAF1 Antibody

Western Blot: PRAF1 Antibody [H00064425-B01P]

Western Blot: PRAF1 Antibody [H00064425-B01P]

Western Blot: PRAF1 Antibody [H00064425-B01P] - Analysis of POLR1E expression in U-2 OS.
Immunocytochemistry/ Immunofluorescence: PRAF1 Antibody [H00064425-B01P]

Immunocytochemistry/ Immunofluorescence: PRAF1 Antibody [H00064425-B01P]

Immunocytochemistry/Immunofluorescence: PRAF1 Antibody [H00064425-B01P] - Analysis of purified antibody to PRAF1 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: PRAF1 Antibody [H00064425-B01P]

Western Blot: PRAF1 Antibody [H00064425-B01P]

Western Blot: PRAF1 Antibody [H00064425-B01P] - Analysis of POLR1E expression in transfected 293T cell line by POLR1E polyclonal antibody. Lane 1: PRAF1 transfected lysate(46.09 KDa). Lane 2: Non-transfected lysate.

Applications for PRAF1 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRAF1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to beinvolved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I andUBTF/UBF

Alternate Names

DNA-directed RNA polymerase I subunit E, DNA-directed RNA polymerase I subunit RPA49, FLJ13390, FLJ13970, FLJ43482, PAF53RNA polymerase I-associated factor 1, polymerase (RNA) I associated factor 1, polymerase (RNA) I polypeptide E, 53kDa, PRAF1, RNA polymerase I associated factor 53, RNA polymerase I subunit A49, RNA polymerase I-associated factor 53, RP11-405L18.3

Entrez Gene IDs

64425 (Human)

Gene Symbol

POLR1E

UniProt

NP_071935.1 (Human)

Additional PRAF1 Products

Product Documents for PRAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRAF1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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