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PRAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29931

Novus Biologicals, part of Bio-Techne
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NBP3-29931

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 431 and 481

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRAF1 Antibody

PRAF1 Antibody

Immunoprecipitation:PRAF1 AntibodyNBP3-29931] -

Immunoprecipitation:PRAF1 AntibodyNBP3-29931] -Detection of human POLR1E/PRAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1E/PRAF1 antibody NBP3-29931 (lot NBP3-29931-1) used for IP at 6 µg per reaction. POLR1E/PRAF1 was also immunoprecipitated by rabbit anti-POLR1E/PRAF1.. For blotting immunoprecipitated POLR1E/PRAF1, NBP3-29931 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PRAF1 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRAF1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to beinvolved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I andUBTF/UBF

Alternate Names

DNA-directed RNA polymerase I subunit E, DNA-directed RNA polymerase I subunit RPA49, FLJ13390, FLJ13970, FLJ43482, PAF53RNA polymerase I-associated factor 1, polymerase (RNA) I associated factor 1, polymerase (RNA) I polypeptide E, 53kDa, PRAF1, RNA polymerase I associated factor 53, RNA polymerase I subunit A49, RNA polymerase I-associated factor 53, RP11-405L18.3

Gene Symbol

POLR1E

Additional PRAF1 Products

Product Documents for PRAF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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